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Enhanced assembly of bacteriophage T7 produced in cell-free reactions under simulated microgravity

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Lehr,  Francois-Xavier
Emmy Noether research Group Cell-free Synthetic Biology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Glatter,  Timo       
Core Facility Mass Spectrometry and Proteomics, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Niederholtmeyer,  Henrike       
Emmy Noether research Group Cell-free Synthetic Biology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;
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Citation

Lehr, F.-X., Pavletić, B., Glatter, T., Heimerl, T., Moeller, R., & Niederholtmeyer, H. (2024). Enhanced assembly of bacteriophage T7 produced in cell-free reactions under simulated microgravity. npj Microgravity, 10: 30. doi:10.1038/s41526-024-00378-4.


Cite as: https://hdl.handle.net/21.11116/0000-000F-1417-1
Abstract
On-demand biomanufacturing has the potential to improve healthcare and self-sufficiency during space missions. Cell-free transcription and translation reactions combined with DNA blueprints can produce promising therapeutics like bacteriophages and virus-like particles. However, how space conditions affect the synthesis and self-assembly of such complex multi-protein structures is unknown. Here, we characterize the cell-free production of infectious bacteriophage T7 virions under simulated microgravity. Rotation in a 2D-clinostat increased the number of infectious particles compared to static controls. Quantitative analyses by mass spectrometry, immuno-dot-blot and real-time PCR showed no significant differences in protein and DNA contents, suggesting enhanced self-assembly of T7 phages in simulated microgravity. While the effects of genuine space conditions on the cell-free synthesis and assembly of bacteriophages remain to be investigated, our findings support the vision of a cell-free synthesis-enabled “astropharmacy”.