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Meeting Abstract

Genetic and Transgenic Approaches in the Nematode Model Pristionchus pacificus

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Sommer,  RJ       
Department Integrative Evolutionary Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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https://www.academia.edu/6817403/Abstract_book_of_5th_International_Congress_of_Nematology_2008
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Citation

Sommer, R. (2008). Genetic and Transgenic Approaches in the Nematode Model Pristionchus pacificus. In 5th International Congress of Nematology (ICN 2008) (pp. 162).


Cite as: https://hdl.handle.net/21.11116/0000-000F-2CCA-D
Abstract
Pristionchus pacificus has been established as a model system in evolutionary biology, in particular in evolutionary developmental biology (evo-devo). P. pacificus shares with Caenorhabditis elegansmany technical features starting form self-fertilizing propagation with the spontaneous generation of males, which allows the use of forward genetic technology. Under laboratory conditions, the life cycle of P. pacificus is 3-4 days (20° C) on Escherichia coli as food source. The genome of P. pacificus has recently been sequenced and is substantially larger than the one of C. elegans or Brugia malayi. Because studies on the regulation of vulva development revealed many important differences between P. pacificus and C. elegans, we have started to establish a comprehensive tool kit for functional studies in these nematodes. In the talk, I will provide an overview on the state of reverse genetics by RNAi and morpholino studies, forward genetics, genomics and DNA-mediated transformation.