Stable Isotope Labeling and Quantification of Photosynthetic Metabolites

Baccolini, C.; Arrivault, S.

doi:10.1007/978-1-0716-3790-6_24

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Stable Isotope Labeling and Quantification of Photosynthetic Metabolites

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Baccolini, C.
Emeritus Group Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Arrivault, S.
Emeritus Group Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Citation

Baccolini, C., & Arrivault, S. (2024). Stable Isotope Labeling and Quantification of Photosynthetic Metabolites. In S. Covshoff (Ed.), Methods and Protocols: Photosynthesis (pp. 439-466). New York: Humana. doi:10.1007/978-1-0716-3790-6_24.

Cite as: https://hdl.handle.net/21.11116/0000-000F-38CA-F

Abstract

Stable isotope labeling with 13CO2 coupled with mass spectrometry allows monitoring the incorporation of 13C into photosynthetic intermediates and is a powerful technique for the investigation of the metabolic dynamics of photosynthesis. We describe here a protocol for 13CO2 labeling of large leaved plants and of Arabidopsis thaliana rosette, and a method for quantitative mass spectrometry analyses to uncover the labeling pattern of Calvin-Benson cycle sucrose, and starch synthesis as well as carbon-concentrating mechanism metabolites.