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Journal Article

Rationally designed chromosome fusion does not prevent rapid growth of Vibrio natriegens

MPS-Authors

Ramming,  Lea
Core Facility MPG MAXGenesys DNAfoundry, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Stukenberg,  Daniel
IMPRS-Mic, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Sanchez Olmos,  Maria del Carmen
Core Facility MPG MAXGenesys DNAfoundry, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Glatter,  Timo       
Core Facility Mass Spectrometry and Proteomics, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Schindler,  Daniel       
Core Facility MPG MAXGenesys DNAfoundry, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

External Resource

https://doi.org/10.1038/s42003-024-06234-1
(Publisher version)

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Citation

Ramming, L., Stukenberg, D., Sanchez Olmos, M. d. C., Glatter, T., Becker, A., & Schindler, D. (2024). Rationally designed chromosome fusion does not prevent rapid growth of Vibrio natriegens. Communications Biology, 7(1): 519. doi:10.1038/s42003-024-06234-1.


Cite as: https://hdl.handle.net/21.11116/0000-000F-3D44-1
Abstract
DNA replication is essential for the proliferation of all cells. Bacterial chromosomes are replicated bidirectionally from a single origin of replication, with replication proceeding at about 1000 bp per second. For the model organism, Escherichia coli, this translates into a replication time of about 40 min for its 4.6 Mb chromosome. Nevertheless, E. coli can propagate by overlapping replication cycles with a maximum short doubling time of 20 min. The fastest growing bacterium known, Vibrio natriegens, is able to replicate with a generation time of less than 10 min. It has a bipartite genome with chromosome sizes of 3.2 and 1.9 Mb. Is simultaneous replication from two origins a prerequisite for its rapid growth? We fused the two chromosomes of V. natriegens to create a strain carrying one chromosome with a single origin of replication. Compared to the parental, this strain showed no significant deviation in growth rate. This suggests that the split genome is not a prerequisite for rapid growth.