Recycling of modified H2A-H2B provides short-term memory of chromatin states

Flury, Valentin; Reverón-Gómez, Nazaret; Alcaraz, Nicolas; Stewart-Morgan, Kathleen R; Wenger, Alice; Klose, Robert J; Groth, Anja

doi:10.1016/j.cell.2023.01.007

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Recycling of modified H2A-H2B provides short-term memory of chromatin states

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Flury, Valentin
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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Flury, V., Reverón-Gómez, N., Alcaraz, N., Stewart-Morgan, K. R., Wenger, A., Klose, R. J., et al. (2023). Recycling of modified H2A-H2B provides short-term memory of chromatin states. Cell, 186, 1050-1065. doi:10.1016/j.cell.2023.01.007.

Cite as: https://hdl.handle.net/21.11116/0000-000F-4491-0

Abstract

Chromatin landscapes are disrupted during DNA replication and must be restored faithfully to maintain genome regulation and cell identity. The histone H3-H4 modification landscape is restored by parental histone recycling and modification of new histones. How DNA replication impacts on histone H2A-H2B is currently unknown. Here, we measure H2A-H2B modifications and H2A.Z during DNA replication and across the cell cycle using quantitative genomics. We show that H2AK119ub1, H2BK120ub1, and H2A.Z are recycled accurately during DNA replication. Modified H2A-H2B are segregated symmetrically to daughter strands via POLA1 on the lagging strand, but independent of H3-H4 recycling. Post-replication, H2A-H2B modification and variant landscapes are quickly restored, and H2AK119ub1 guides accurate restoration of H3K27me3. This work reveals epigenetic transmission of parental H2A-H2B during DNA replication and identifies cross talk between H3-H4 and H2A-H2B modifications in epigenome propagation. We propose that rapid short-term memory of recycled H2A-H2B modifications facilitates restoration of stable H3-H4 chromatin states.