Short-time phosphorylation of plasma membrane proteins by endogenous kinases

Joos, T; Anderer, FA

doi:10.1016/0006-291x(78)90756-8

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Short-time phosphorylation of plasma membrane proteins by endogenous kinases

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Joos, T
Anderer Group, Friedrich Miescher Laboratory, Max Planck Society;

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Anderer, FA
Anderer Group, Friedrich Miescher Laboratory, Max Planck Society;

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Joos, T., & Anderer, F. (1978). Short-time phosphorylation of plasma membrane proteins by endogenous kinases. Biochemical and Biophysical Research Communications, 84(3), 660-666. doi:10.1016/0006-291x(78)90756-8.

Cite as: https://hdl.handle.net/21.11116/0000-000F-508F-6

Abstract

Endogenous protein phosphorylation in plasma membranes isolated from SV 40-transformed mouse fibroblasts was studied in the presence and absence of cyclic nucleotides. Using low concentrations of membrane protein the kinetics of ATP-dependent 32P-incorporation showed a rapid phosphorylation reaction up to 2 sec of incubation which was stimulated by cAMP and inhibited by cGMP. This short-time phosphorylation reaction was followed by a rapid dephosphorylation and a slower rephosphorylation. This phenomenon was dependent on protein concentration.