FACT maintains chromatin architecture and thereby stimulates RNA polymerase II 
pausing during transcription in vivo

Žumer, Kristina; Ochmann, Moritz; Aljahani, Abrar; Zheenbekova, Aiturgan; Devadas, Arjun; Maier, Kerstin C.; Rus, Petra; Neef, Ute; Oudelaar, Aukje Marieke; Cramer, Patrick

doi:10.1016/j.molcel.2024.05.003

Datensatz

DATENSATZ AKTIONENEXPORT

Zur Ablage hinzufügen

Lokale TagsFreigabegeschichteDetailsÜbersicht

Freigegeben

Zeitschriftenartikel

FACT maintains chromatin architecture and thereby stimulates RNA polymerase II pausing during transcription in vivo

MPG-Autoren

/persons/resource/persons199330

Žumer, Kristina
Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons248419

Ochmann, Moritz
Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons252095

Aljahani, Abrar
Lise Meitner Group Genome Organization and Regulation, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons298756

Zheenbekova, Aiturgan
Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons298758

Devadas, Arjun
Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons199094

Maier, Kerstin C.
Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons203891

Rus, Petra
Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons252091

Oudelaar, Aukje Marieke
Lise Meitner Group Genome Organization and Regulation, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons127020

Cramer, Patrick
Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

Externe Ressourcen

Es sind keine externen Ressourcen hinterlegt

Volltexte (beschränkter Zugriff)

Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.

Volltexte (frei zugänglich)

Publisher Version
(Verlagsversion), 8MB

Ergänzendes Material (frei zugänglich)

Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar

Zitation

Žumer, K., Ochmann, M., Aljahani, A., Zheenbekova, A., Devadas, A., Maier, K. C., et al. (2024). FACT maintains chromatin architecture and thereby stimulates RNA polymerase II pausing during transcription in vivo. Molecular Cell, 84(11), 2053-2069.e9. doi:10.1016/j.molcel.2024.05.003.

Zitierlink: https://hdl.handle.net/21.11116/0000-000F-5BF8-4

Zusammenfassung

Facilitates chromatin transcription (FACT) is a histone chaperone that supports transcription through chromatin in vitro, but its functional roles in vivo remain unclear. Here, we analyze the in vivo functions of FACT with the use of multi-omics analysis after rapid FACT depletion from human cells. We show that FACT depletion destabilizes chromatin and leads to transcriptional defects, including defective promoter-proximal pausing and elongation, and increased premature termination of RNA polymerase II. Unexpectedly, our analysis revealed that promoter-proximal pausing depends not only on the negative elongation factor (NELF) but also on the +1 nucleosome, which is maintained by FACT.