From genes to protein mechanics on a chip

Otten, Marcus; Ott, Wolfgang; Jobst, Markus A; Milles, Lukas F.; Verdorfer, Tobias; Pippig, Diana A; Nash, Michael A; Gaub, Hermann E

doi:10.1038/nmeth.3099

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

From genes to protein mechanics on a chip

MPS-Authors

There are no MPG-Authors in the publication available

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Otten, M., Ott, W., Jobst, M. A., Milles, L. F., Verdorfer, T., Pippig, D. A., et al. (2014). From genes to protein mechanics on a chip. Nature Methods, (11), 1127-1130. doi:10.1038/nmeth.3099.

Cite as: https://hdl.handle.net/21.11116/0000-000F-8364-C

Abstract

Single-molecule force spectroscopy enables mechanical testing of individual proteins, but low experimental throughput limits the ability to screen constructs in parallel. We describe a microfluidic platform for on-chip expression, covalent surface attachment and measurement of single-molecule protein mechanical properties. A dockerin tag on each protein molecule allowed us to perform thousands of pulling cycles using a single cohesin-modified cantilever. The ability to synthesize and mechanically probe protein libraries enables high-throughput mechanical phenotyping.