A chinrest-based approach to measure eye movements and experimental task 
engagement in macaques with minimal restraint

Rima, Samy; Greilsamer, Jennifer; Haag, Marcus; Cadena-Valencia, Jaime; Sansonnens, Morgan; Francovich, Andrea; Lanz, Florian; Zbinden, Andrina; Bergadano, Alessandra; Schmid, Michael Christoph

doi:10.1016/j.jneumeth.2024.110173

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Journal Article

A chinrest-based approach to measure eye movements and experimental task engagement in macaques with minimal restraint

MPS-Authors

Schmid, Michael Christoph
Ernst Strüngmann Institute (ESI) for Neuroscience in Cooperation with Max Planck Society, Max Planck Society;

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https://www.sciencedirect.com/science/article/pii/S0165027024001183
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Rima_2024_AChinrest-basedApproach.pdf
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Citation

Rima, S., Greilsamer, J., Haag, M., Cadena-Valencia, J., Sansonnens, M., Francovich, A., et al. (2024). A chinrest-based approach to measure eye movements and experimental task engagement in macaques with minimal restraint. Journal of Neuroscience Methods, 408: 110173. doi:10.1016/j.jneumeth.2024.110173.

Cite as: https://hdl.handle.net/21.11116/0000-000F-85EE-F

Abstract

Highlights

• This study introduces a transformative approach to macaque biomedical research using a 3D-printed chinrest adapted from canine research, enhancing both precision and animal welfare.
• The chinrest ensures accurate eye movement measurements for cognitive function studies while allowing the macaques to autonomously engage with or withdraw from experiments.
• Findings show the chinrest provides comparable or enhanced performance over traditional immobilization techniques, with significant implications for non-human primate research.
• This method promotes more ethical research practices and improves training efficiency, balancing scientific rigor with animal welfare advancements.

Abstract

Background
The use of Rhesus macaques in vision research is crucial due to their visual system's similarity to humans. While invasive techniques have been the norm, there has been a shift towards non-invasive methods, such as facemasks and head molds, to enhance animal welfare and address ethical concerns.

New Method
We present a non-invasive, 3D-printed chinrest with infrared sensors, adapted from canine research, allowing for accurate eye movement measurements and voluntary animal participation in experiments.

Results
The chinrest method showed a 16% and 28% increase in average trial numbers for Monkey 1 and Monkey 2, respectively, compared to the traditional headpost method. The engagement was high, with monkeys performing over 500 trials per session and initiating a new trial after an average intertrial interval of approximately 1 second. The hit rate improved by about 10% for Monkey 1 in the chinrest condition, and the fixation precision, measured by the standard deviation of gaze positions, was significantly better in the chinrest condition, with Monkey 1 showing a reduction in fixation imprecision from 0.26° to 0.17° in the X-axis.

Comparison with Existing Methods
The chinrest approach showed significant improvements in trial engagement and reduction in aborted trials due to fixation breaks, indicating less stress and potentially improved data quality compared to previous non-invasive methods.

Conclusions
The chinrest method offers a significant advancement in primate cognitive testing by allowing for precise data collection while addressing animal welfare concerns, possibly leading to better scientific outcomes and a paradigm shift in primate research methodologies.