Modular Assembly of Synthetic Secondary Chromosomes

Zumkeller, Celine; Schindler, Daniel; Felder, Jennifer; Waldminghaus, Torsten

doi:10.1007/978-1-0716-3930-6_9

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Modular Assembly of Synthetic Secondary Chromosomes

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Schindler, Daniel
Core Facility MPG MAXGenesys DNAfoundry, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Citation

Zumkeller, C., Schindler, D., Felder, J., & Waldminghaus, T. (2024). Modular Assembly of Synthetic Secondary Chromosomes. In R. T. Dame (Ed.), Bacterial Chromatin. Methods in Molecular Biology, (2819, pp. 157-187). Humana, New York, NY.

Cite as: https://hdl.handle.net/21.11116/0000-000F-9768-2

Abstract

The development of novel DNA assembly methods in recent years has paved the way for the construction of synthetic replicons to be used for basic research and biotechnological applications. A learning-by-building approach can now answer questions about how chromosomes must be constructed to maintain genetic information. Here we describe an efficient pipeline for the design and assembly of synthetic, secondary chromosomes in Escherichia coli based on the popular modular cloning (MoClo) system.