Function of coenzyme F420-dependent NADP reductase in methanogenic archaea 
containing an NADP-dependent alcohol dehydrogenase

Berk, Holger; Thauer, Rudolf K.

doi:10.1007/s002030050514

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Function of coenzyme F₄₂₀-dependent NADP reductase in methanogenic archaea containing an NADP-dependent alcohol dehydrogenase

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Thauer, Rudolf K.
Department of Biochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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https://doi.org/10.1007/s002030050514
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Citation

Berk, H., & Thauer, R. K. (1997). Function of coenzyme F₄₂₀-dependent NADP reductase in methanogenic archaea containing an NADP-dependent alcohol dehydrogenase. Archives of Microbiology, 168(5), 396-402. doi:10.1007/s002030050514.

Cite as: https://hdl.handle.net/21.11116/0000-000F-AC23-8

Abstract

Methanogenic archaea growing on ethanol or isopropanol as the electron donor for CO2 reduction to CH4 contain either an NADP-dependent or a coenzyme F420-dependent alcohol dehydrogenase. We report here that in both groups of methanogens, the N5, N10-methylenetetrahydromethanopterin dehydrogenase and the N5, N10-methylenetetrahydromethanopterin reductase, two enzymes involved in CO2 reduction to CH4, are specific for F420. This raised the question how F420H2 is regenerated in the methanogens with an NADP-dependent alcohol dehydrogenase. We found that these organisms contain catabolic activities of an enzyme catalyzing the reduction of F420 with NADPH. The F420-dependent NADP reductase from Methanogenium organophilum was purified and characterized. The N-terminal amino acid sequence showed 42% sequence identity to a putative gene product in Methanococcus jannaschii, the total genome of which has recently been sequenced.