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Journal Article

Multiarray cell stretching platform for high-magnification real-time imaging

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Citation

Huang, Y., Nam-Trung, N., Lok, K. S., Lee, P. P. F., Su, M., Wu, M., et al. (2013). Multiarray cell stretching platform for high-magnification real-time imaging. Nanomedicine, 8(4), 543-553. doi:10.2217/NNM.13.45.


Cite as: https://hdl.handle.net/21.11116/0000-000F-B599-8
Abstract
Aim: This article reports the development of a multiarray microchip with real-time imaging capability to apply mechanical strains onto monolayered cell cultures. Materials & methods: Cells were cultured on an 8-mu m thick membrane that was positioned in the microscope focal plane throughout the stretching process. Each stretching unit was assembled from three elastomeric layers and a glass coverslip. A programmable pneumatic control system was developed to actuate this platform. Multiple stretching experiments were conducted with various cell lines. Results: The platform provides a maximum uniform strain of 69%. Acute and long-term cell morphological changes were observed. The supreme imaging capability was verified by real-time imaging of transfected COS-7 stretching and poststretching imaging of immunofluorescence-stained PTK2. Conclusion: The platform reported here is a powerful tool for studying mechanically induced physiological changes in cells. Such a device could be used in tissue regeneration for maintaining essential cell growth conditions.