Fast kinetics of chromatin assembly revealed by single-molecule videomicroscopy 
and scanning force microscopy

Ladoux, B.; Quivy, JP; Doyle, P; du Roure, O; Almouzni, G; Viovy, JL

doi:10.1073/pnas.250471597

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Fast kinetics of chromatin assembly revealed by single-molecule videomicroscopy and scanning force microscopy

MPS-Authors

There are no MPG-Authors in the publication available

External Resource

https://www.pnas.org/doi/10.1073/pnas.250471597
(Publisher version)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Ladoux, B., Quivy, J., Doyle, P., du Roure, O., Almouzni, G., & Viovy, J. (2000). Fast kinetics of chromatin assembly revealed by single-molecule videomicroscopy and scanning force microscopy. PNAS, 97(26), 14251-14256. doi:10.1073/pnas.250471597.

Cite as: https://hdl.handle.net/21.11116/0000-000F-B523-D

Abstract

Fluorescence videomicroscopy and scanning force microscopy were used to follow, in Peal time, chromatin assembly on individual DNA molecules immersed in cell-free systems competent for physiological chromatin assembly. Within a few seconds, molecules are already compacted into a form exhibiting strong similarities to native chromatin fibers. In these extracts, the compaction rate is more than 100 times faster than expected from standard biochemical assays. Our data provide definite information on the forces involved (a few piconewtons) and on the reaction path. DNA compaction as a function of time revealed unique features of the assembly reaction in these extracts. They imply a sequential process with at least three steps, involving DNA wrapping as the final event. An absolute and quantitative measure of the kinetic parameters of the early steps in chromatin assembly under physiological conditions could thus be obtained.