English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Book Chapter

Lipid and protein mobility in giant unilamellar vesicles

MPS-Authors
There are no MPG-Authors in the publication available
External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Ugarte-Uribe, B., Das, K. K., & García-Sáez, A. J. (2019). Lipid and protein mobility in giant unilamellar vesicles. In The Giant Vesicle Book. CRC Press.


Cite as: https://hdl.handle.net/21.11116/0000-000F-B86A-B
Abstract
The combination of giant unilamellar vesicles (GUVs) and current fluorescent-based technologies provide an efficient platform for the study of membranes in terms of the presence and size of membrane domains, lipid-protein and protein-protein interactions within the membrane, and additional factors that can directly alter the lateral mobility of lipids. Hence, this chapter focuses on fluorescence-based approaches involved in broad understanding of the dynamic properties of biological membranes by using a simple and biochemically well-defined system such as GUVs. In particular, the principles and applications of fluorescence correlation spectroscopy (FCS), fluorescence recovery after photobleaching (FRAP) and single-molecule imaging (SMI) are described, as well as the current limitations which are expected to be overcome during the next years.