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Journal Article

Inhibition of methane oxidation by ammonium in the surface layer of a littoral sediment

MPS-Authors

Bosse,  U.
Department of Biogeochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Frenzel,  Peter
Department of Biogeochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Conrad,  Ralf       
Department of Biogeochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Citation

Bosse, U., Frenzel, P., & Conrad, R. (1993). Inhibition of methane oxidation by ammonium in the surface layer of a littoral sediment. FEMS Microbiology Ecology, 13(2), 123-134. doi:10.1111/j.1574-6941.1993.tb00058.x.


Cite as: https://hdl.handle.net/21.11116/0000-000F-BF9B-C
Abstract
The diffusive flux of CH4 from the sediment into the water was measured in intact sediment cores taken from the littoral of Lake Constance. CH4 fluxes were measured under oxic and anoxic conditions. Emissions under anoxic conditions were used to estimate the potential diffusive flux. The results showed that about 79% of the CH4 diffusing upwards was oxidized at the sediment surface. Kinetic measurements of CH4 oxidation in diluted surface sediment gave V-max = 1.2 mu mol CH4 ml(sed)(-1)h(-1). This was high enough to explain the oxidation rates calculated from the flux measurements. NH4+ was added to the water overlaying the sediment cores to measure its influence on CH4 oxidation. This treatment resulted in increased concentrations of pore water NH4+ which were measured with a NH4+ microelectrode. Pore water NH4+ concentrations of < 4 mM had no effect on CH4 oxidation while concentrations between 4 and 10 mM NH4+ reduced the CH4 oxidation rates by about 30%. Measurement of CH4 oxidation rates in diluted surface sediment gave similar results. At CH4 concentrations of 21 mu M, the CH4 oxidation rate decreased at NH4+ concentrations greater than or equal to 4 mM, and was completely inhibited at NH4+ concentrations > 20 mM. Measurements of potential NH4+ oxidation rates were below the detection limit of 0.5 nmol NO2- produced ml(sed)(-1)h(-1). Thus, the NH4+ oxidizing activity was about 3 magnitudes lower than the CH4 oxidizing activity, and it is unlikely that NH4+ oxidizers are important for CH4 oxidation in this littoral sediment.