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Demonstration of two different H2-oxidizing activities in soil using a H2 consumption and a tritium exchange assay

MPS-Authors

HARING,  V
Department of Biogeochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Conrad,  R       
Department of Biogeochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Citation

HARING, V., & Conrad, R. (1994). Demonstration of two different H2-oxidizing activities in soil using a H2 consumption and a tritium exchange assay. Biology and Fertility of Soils, 17(2), 125-128. doi:10.1007/BF00337744.


Cite as: https://hdl.handle.net/21.11116/0000-000F-C9E5-C
Abstract
H-2-oxidizing activities were assayed in slurries of four soils by measuring the consumption of H-2 and the exchange of H-3(2) with H2O at increasing mixing ratios of H-2 or H-3(2). Both H-2 consumption and H-3(2) exchange were abolished by autoclaving or the addition of formaldehyde. The rates of H-2 Consumption and H-3(2) exchange were proportional to the quantity of soil used. Both activities increased with increasing concentrations of H-2 or H-3(2) and displayed biphasic kinetics, demonstrating the existence of two different H-2-oxidizing activities, one with a relatively low K(m) and V(max), and a second with a relatively high K(m) and V(max). The first type of activity was characteristic of abiontic soil hydrogenases, and the second of aerobic H-2-oxidizing bacteria. In contrast to H-2 consumption, which required the presence of either 02 or ferricyanide, H-3(2) exchange operated equally well without an external electron acceptor. The H-3(2) exchange assay may thus be particularly useful for enrichment of soil hydrogenases which have not yet been isolated and for which no natural electron acceptor is known.