English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Lipid Membrane Topographies Are Regulators for the Spatial Distribution of Liquid Protein Condensates

MPS-Authors
/persons/resource/persons81106

Zieske,  Katja
Zieske Research Group, Research Groups, Max Planck Institute for the Science of Light, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Supplementary Material (public)
There is no public supplementary material available
Citation

Kang, C. Y., Chang, Y., & Zieske, K. (2024). Lipid Membrane Topographies Are Regulators for the Spatial Distribution of Liquid Protein Condensates. Nano Letters, 24, 4330-4335. doi:10.1021/acs.nanolett.3c04169.


Cite as: https://hdl.handle.net/21.11116/0000-000F-D24C-F
Abstract
Liquid protein condensates play important roles in orchestrating subcellular organization and as biochemical reaction hubs. Recent studies have linked lipid membranes to proteins capable of forming liquid condensates, and shown that biophysical parameters, like protein enrichment and restricted diffusion at membranes, regulate condensate formation and size. However, the impact of membrane topography on liquid condensates remains poorly understood. Here, we devised a cell-free system to reconstitute liquid condensates on lipid membranes with microstructured topographies and demonstrated that lipid membrane topography is a significant biophysical regulator. Using membrane surfaces designed with microwells, we observed ordered condensate patterns. Furthermore, we demonstrate that membrane topographies influence the shape of liquid condensates. Finally, we show that capillary forces, mediated by membrane topographies, lead to the directed fusion of liquid condensates. Our results demonstrate that membrane topography is a potent biophysical regulator for the localization and shape of mesoscale liquid protein condensates.