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Journal Article

Photonic neural probe enabled microendoscopes for light-sheet light-field computational fluorescence brain imaging

MPS-Authors
/persons/resource/persons291494

Ding,  Peisheng
Nanophotonics, Integration, and Neural Technology, Max Planck Institute of Microstructure Physics, Max Planck Society;

/persons/resource/persons265922

Wahn,  Hannes
Nanophotonics, Integration, and Neural Technology, Max Planck Institute of Microstructure Physics, Max Planck Society;

/persons/resource/persons260636

Chen,  Fu-Der       
Nanophotonics, Integration, and Neural Technology, Max Planck Institute of Microstructure Physics, Max Planck Society;
Max Planck - University of Toronto Centre for Neural Science and Technology, Max Planck Institute of Microstructure Physics, Max Planck Society;

/persons/resource/persons283203

Li,  Jianfeng       
Nanophotonics, Integration, and Neural Technology, Max Planck Institute of Microstructure Physics, Max Planck Society;
Max Planck - University of Toronto Centre for Neural Science and Technology, Max Planck Institute of Microstructure Physics, Max Planck Society;

/persons/resource/persons265928

Mu,  Xin       
Nanophotonics, Integration, and Neural Technology, Max Planck Institute of Microstructure Physics, Max Planck Society;
Max Planck - University of Toronto Centre for Neural Science and Technology, Max Planck Institute of Microstructure Physics, Max Planck Society;

/persons/resource/persons265926

Stalmashonak,  Andrei
Nanophotonics, Integration, and Neural Technology, Max Planck Institute of Microstructure Physics, Max Planck Society;

/persons/resource/persons257612

Poon,  Joyce K. S.       
Nanophotonics, Integration, and Neural Technology, Max Planck Institute of Microstructure Physics, Max Planck Society;
Max Planck - University of Toronto Centre for Neural Science and Technology, Max Planck Institute of Microstructure Physics, Max Planck Society;

/persons/resource/persons258003

Sacher,  Wesley D.       
Nanophotonics, Integration, and Neural Technology, Max Planck Institute of Microstructure Physics, Max Planck Society;
Max Planck - University of Toronto Centre for Neural Science and Technology, Max Planck Institute of Microstructure Physics, Max Planck Society;

External Resource

https://doi.org/10.1117/1.NPh.11.S1.S11503
(Publisher version)

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Fulltext (public)

S11503_1-1.pdf
(Publisher version), 6MB

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Citation

Ding, P., Wahn, H., Chen, F.-D., Li, J., Mu, X., Stalmashonak, A., et al. (2024). Photonic neural probe enabled microendoscopes for light-sheet light-field computational fluorescence brain imaging. Neurophotonics, 11(S1): S11503. doi:10.1117/1.NPh.11.S1.S11503.


Cite as: https://hdl.handle.net/21.11116/0000-000F-D8B4-2
Abstract
Significance
Light-sheet fluorescence microscopy is widely used for high-speed, high-contrast, volumetric imaging. Application of this technique to in vivo brain imaging in non-transparent organisms has been limited by the geometric constraints of conventional light-sheet microscopes, which require orthogonal fluorescence excitation and collection objectives. We have recently demonstrated implantable photonic neural probes that emit addressable light sheets at depth in brain tissue, miniaturizing the excitation optics. Here, we propose a microendoscope consisting of a light-sheet neural probe packaged together with miniaturized fluorescence collection optics based on an image fiber bundle for lensless, light-field, computational fluorescence imaging.

Aim
Foundry-fabricated, silicon-based, light-sheet neural probes can be packaged together with commercially available image fiber bundles to form microendoscopes for light-sheet light-field fluorescence imaging at depth in brain tissue.

Approach
Prototype microendoscopes were developed using light-sheet neural probes with five addressable sheets and image fiber bundles. Fluorescence imaging with the microendoscopes was tested with fluorescent beads suspended in agarose and fixed mouse brain tissue.

Results
Volumetric light-sheet light-field fluorescence imaging was demonstrated using the microendoscopes. Increased imaging depth and enhanced reconstruction accuracy were observed relative to epi-illumination light-field imaging using only a fiber bundle.
Conclusions

Our work offers a solution toward volumetric fluorescence imaging of brain tissue with a compact size and high contrast. The proof-of-concept demonstrations herein illustrate the operating principles and methods of the imaging approach, providing a foundation for future investigations of photonic neural probe enabled microendoscopes for deep-brain fluorescence imaging in vivo.