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Journal Article

Stimulated emission depletion microscopy

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Lukinavičius,  Gražvydas
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;
Research Group of Chromatin Labeling and Imaging, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Gerasimaitė,  Rūta
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;
Research Group of Chromatin Labeling and Imaging, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Nguyễn,  Văn Thắng       
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;
Research Group of Chromatin Labeling and Imaging, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Citation

Lukinavičius, G., Alvelid, J., Gerasimaitė, R., Rodilla-Ramirez, C., Nguyễn, V. T., Vicidomini, G., et al. (2024). Stimulated emission depletion microscopy. Nature Reviews Methods primers, 4(1): 56. doi:10.1038/s43586-024-00335-1.


Cite as: https://hdl.handle.net/21.11116/0000-000F-EF71-5
Abstract
In this Primer, we focus on the most recent advancements in stimulated emission depletion (STED) microscopy, encompassing optics, computational microscopy and probes design, which enable STED imaging to open new observation windows in challenging samples such as living cells and tissues. We showcase applications in which STED data have been essential to gain new biological insights in various cell types and model systems. Finally, we discuss what standardization will be important in our view to further advance STED imaging, including open and shareable software, analysis pipelines, data repositories and sample preparation protocols.