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Book Chapter

Cellular Microbiochemistry

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Scheel,  J
Department Physical Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Pepperkok, R., Rosorius, O., & Scheel, J. (1998). Cellular Microbiochemistry. In A. Cid-Arregui, & A. García-Carrancá (Eds.), Microinjection and Transgenesis (pp. 145-154). Berlin, Germany: Spinger.


Cite as: https://hdl.handle.net/21.11116/0000-000F-E156-2
Abstract
Capillary microinjection is efficiently used to introduce macromolecules into the nucleus or cytoplasm of living mammalian cells (Proctor 1992). Transfer can occur at well-defined stages of the cell cycle, and modifications of culture conditions are possible before, during, and after injection. The number of cells which can be injected per experiment is, however, limited. Therefore, in the past, biochemical analyses of microinjected cells has been possible, but difficult (Gautier-Rouviere et al. 1990; Lane et al. 1993).