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Probing the modulation of enzyme kinetics by multi-temperature, time-resolved serial crystallography

MPS-Authors
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Schulz,  E.-C.
University Medical Center Hamburg-Eppendorf (UKE);
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Institute for Nanostructure and Solid State Physics, University of Hamburg;

/persons/resource/persons241300

Leimkohl,  J.-P.
Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

/persons/resource/persons241303

Schikora,  H.
Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

/persons/resource/persons182596

Tellkamp,  F.
Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

/persons/resource/persons209117

Mehrabi,  P.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Institute for Nanostructure and Solid State Physics, University of Hamburg;

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https://doi.org/10.1101/2021.11.07.467596
(Preprint)

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Citation

Schulz, E.-C., Prester, A., von Stetten, D., Gore, G., Hatton, C. E., Bartels, K., et al. (2024). Probing the modulation of enzyme kinetics by multi-temperature, time-resolved serial crystallography. doi:10.1101/2021.11.07.467596.


Cite as: https://hdl.handle.net/21.11116/0000-000F-FB2B-7
Abstract
We present an environmental enclosure for fixed-target serial crystallography, enabling X-ray diffraction experiments in a temperature window from below 10 °C to above 70 °C - a universal parameter of protein function. Via 5D-SSX time-resolved experiments can now be carried out at physiological temperatures, providing fundamentally new insights into protein function. We show temperature-dependent modulation of turnover kinetics for the mesophilic β-lactamase CTX-M-14 and for the hyperthermophilic enzyme xylose isomerase.