English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Optimizing Cryo-FIB Lamellas for sub-5Å in situ Structural Biology

MPS-Authors
/persons/resource/persons265783

Khavnekar,  Sagar
Plitzko, Jürgen / Cryo-EM Technology, Max Planck Institute of Biochemistry, Max Planck Society;

Vrbovská,  Veronika
Max Planck Society;

Zaoralová,  Magda
Max Planck Society;

Kelley,  Ron
Max Planck Society;

/persons/resource/persons77726

Beck,  Florian
Plitzko, Jürgen / Cryo-EM Technology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons249307

Klumpe,  Sven
Plitzko, Jürgen / Cryo-EM Technology, Max Planck Institute of Biochemistry, Max Planck Society;

Kotecha,  Abhay
Max Planck Society;

/persons/resource/persons78517

Plitzko,  Jürgen
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

Erdmann,  Philipp S.
Max Planck Society;

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Khavnekar, S., Vrbovská, V., Zaoralová, M., Kelley, R., Beck, F., Klumpe, S., et al. (2022). Optimizing Cryo-FIB Lamellas for sub-5Å in situ Structural Biology. bioRxiv: the preprint server for biology. doi:10.1101/2022.06.16.496417.


Cite as: https://hdl.handle.net/21.11116/0000-0010-33B3-A
Abstract
We here present a method based on metallic platinum sputtering that can substantially enhance the quality of subtomogram averages from lamellas and thereby reduce the number of particles needed for high-resolution subtomogram averaging. We provide evidence for the physical background of this improvement and demonstrate its usefulness by producing sub-5Å ribosome averages from yeast.Competing Interest StatementV.V., M.Z., R.K. and A.K. are employees of Thermo Fisher Scientific. The other authors declare no competing interests.