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Book Chapter

Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT

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Strauss,  Sebastian
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

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Jungmann,  Ralf
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Strauss, S., & Jungmann, R. (2023). Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT. In G. Mayer, & M. M. Menger (Eds.), Nucleic Acid Aptamers: Selection, Characterization, and Application (pp. 177-185). New York, NY: Springer US.


Cite as: https://hdl.handle.net/21.11116/0000-0010-39A2-7
Abstract
Super-resolution microscopy methods enable the visualization of biological processes on the level of a few nanometers. However, the application of these techniques in biological systems is limited by the availability of small affinity reagents. Slow off-rate-modified aptamers as nucleic acid analogues to antibodies have been successfully applied to improve the resolution and quantification of DNA-PAINT. In this chapter, we describe a protocol for using SOMAmers as labeling reagents for super-resolution microscopy.