One-carbon fixation via the synthetic reductive glycine pathway exceeds yield 
of the Calvin cycle

Dronsella, B.; Orsi, E.; Schulz-Mirbach, Helena; Benito-Vaquerizo, Sara; Yilmaz, Suzan; Glatter, Timo; Bar-Even, A.; Erb, Tobias J.; Claassens, Nico J.

doi:10.1038/s41564-025-01941-9

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One-carbon fixation via the synthetic reductive glycine pathway exceeds yield of the Calvin cycle

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Dronsella, B.
Systems and Synthetic Metabolism, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Orsi, E.
Systems and Synthetic Metabolism, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Bar-Even, A.
Systems and Synthetic Metabolism, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Citation

Dronsella, B., Orsi, E., Schulz-Mirbach, H., Benito-Vaquerizo, S., Yilmaz, S., Glatter, T., et al. (2025). One-carbon fixation via the synthetic reductive glycine pathway exceeds yield of the Calvin cycle. Nature Microbiology, 10, 646-653. doi:10.1038/s41564-025-01941-9.

Cite as: https://hdl.handle.net/21.11116/0000-0010-CC85-2

Abstract

One-carbon feedstocks such as formate could be promising renewable substrates for sustainable microbial production of food, fuels and chemicals. Here we replace the native energy-inefficient Calvin–Benson–Bassham cycle in Cupriavidus necator with the more energy-efficient reductive glycine pathway for growth on formate and CO2. In chemostats, our engineered strain reached a 17% higher biomass yield than the wild type and a yield higher than any natural formatotroph using the Calvin cycle. This shows the potential of synthetic metabolism to realize sustainable, bio-based production.