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A method for trapping intermediates of polyketide biosynthesis with a nonhydrolyzable malonyl-coenzyme A analogue

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Spiteller,  D.
Department of Bioorganic Chemistry, MPI for Chemical Ecology, Max Planck Society;

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Spiteller, D., Waterman, C. L., & Spencer, J. B. (2005). A method for trapping intermediates of polyketide biosynthesis with a nonhydrolyzable malonyl-coenzyme A analogue. Angewandte Chemie International Edition, 44(43), 7079-7082. doi:10.1002/anie.200501670.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0012-A39E-9
Abstract
Trapped in the middle: To investigate the mechanistic details of polyketide biosynthesis, intermediates are trapped by a nonhydrolyzable malonyl‐coenzyme A analogue that reacts with a growing polyketide chain formed by a stilbene synthase. As the reverse transesterification reaction is not possible, the polyketide intermediates accumulate attached to the analogue (see scheme; Enz=enzyme, CoA=coenzyme A).