Pentalenene synthase. Analysis of active site residues by site- directed 
mutagenesis

Seemann, M.; Zhai, G. Z.; Kraker, De; Paschall, C. M.; Christianson, D. W.; Cane, D. E.

doi:10.1021/ja026058q

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Pentalenene synthase. Analysis of active site residues by site- directed mutagenesis

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Kraker, De
Department of Biochemistry, Prof. J. Gershenzon, MPI for Chemical Ecology, Max Planck Society;

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Citation

Seemann, M., Zhai, G. Z., Kraker, D., Paschall, C. M., Christianson, D. W., & Cane, D. E. (2002). Pentalenene synthase. Analysis of active site residues by site- directed mutagenesis. Journal of the American Chemical Society, 124(26), 7681-7689. doi:10.1021/ja026058q.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0012-A792-7

Abstract

Incubation of farnesyl diphosphate (1) with the W308F or W308F/H309F mutants of pentalenene synthase, an enzyme from Streptomyces UC5319, yielded pentalenene (2), accompanied by varying proportions of (+)-germacrene A (7) with relatively minor changes i