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Journal Article

Structure-function relationship of bifunctional scorpion toxin BmBKTx1


Wicher,  Dieter
Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society;

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Wang, S., Huang, L., Wicher, D., Chi, C., & Xu, C. (2008). Structure-function relationship of bifunctional scorpion toxin BmBKTx1. Acta biochimica et biophysica Sinica, 40(11), 955-963. doi:10.1111/j.1745-7270.2008.00479.x.

Cite as: http://hdl.handle.net/11858/00-001M-0000-0012-A938-1
As the first identified scorpion toxin active on both big conductance Ca2+-activated K+ channels (BK) and small conductance Ca2+-activated K+ channels (SK), BmBKTx1 has been proposed to have two separate functional faces for two targets. To investigate this hypothesis, two double mutants, K21A-Y30A and R9A-K11A, together with wild-type toxin were expressed in Escherichia coli. The recombinant toxins were tested on cockroach BK and rat SK2 channel for functional assay. Mutant K21A-Y30A had a dramatic loss of function on BK but retained its function on SK. Mutant R9A-K11A did not lose function on BK or SK. These data support the two functional-face hypothesis and indicate that the BK face is on the C-terminal β-sheet.