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Rapid HPLC screening of jasmonate-induced increases in tobacco alkaloids, phenolics, and diterpene glycosides in Nicotiana attenuata

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Keinänen,  Markku
Department of Molecular Ecology, MPI for Chemical Ecology, Max Planck Society;

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Oldham,  N. J.
Research Group Mass Spectrometry, MPI for Chemical Ecology, Max Planck Society;

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Baldwin,  I. T.
Department of Molecular Ecology, MPI for Chemical Ecology, Max Planck Society;

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Citation

Keinänen, M., Oldham, N. J., & Baldwin, I. T. (2001). Rapid HPLC screening of jasmonate-induced increases in tobacco alkaloids, phenolics, and diterpene glycosides in Nicotiana attenuata. Journal of Agricultural and Food Chemistry, 49(8), 3553-3558. doi:10.1021/jf010200+.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0012-B32B-E
Abstract
A rapid, HPLC-based screening procedure for the main classes of secondary metabolites in Nicotiana attenuata leaves (alkaloids, phenolics, and diterpene glycosides) is reported. In a single step, leaves are extracted in aqueous acidified (0.5% acetic ac