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Journal Article

Probing secondary structures of spin-labeled RNA by pulsed EPR spectroscopy.

MPS-Authors
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Sicoli,  G.
Research Group of Electron Paramagnetic Resonance, MPI for biophysical chemistry, Max Planck Society;

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Bennati,  M.
Research Group of Electron Paramagnetic Resonance, MPI for biophysical chemistry, Max Planck Society;

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Hoebartner,  C.
Research Group of Nucleic Acid Chemistry, MPI for biophysical chemistry, Max Planck Society;

External Resource

http://onlinelibrary.wiley.com/doi/10.1002/anie.201000713/epdf
(Publisher version)

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Fulltext (public)

587641.pdf
(Publisher version), 532KB

Supplementary Material (public)

587641-Suppl.pdf
(Supplementary material), 721KB

Citation

Sicoli, G., Wachowius, F., Bennati, M., & Hoebartner, C. (2010). Probing secondary structures of spin-labeled RNA by pulsed EPR spectroscopy. Angewandte Chemie International Edition, 49(36), 6443-6447. doi:10.1002/anie.201000713.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0012-D545-6
Abstract
Tempo, TEMPO! Spin-labeled RNAs are readily prepared by postsynthetic modification of RNA. This approach provides access to paramagnetic oligonucleotides with position-specific probes in cytidine, adenosine, and guanosine nucleobases (CT, AT, GT, respectively). In this way alternative RNA secondary structures can be investigated by distance measurements using pulsed EPR spectroscopy.