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Journal Article

Exon definition complexes contain the tri-snRNP and can be directly converted into B-like precatalytic splicing complexes.

MPS-Authors
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Schneider,  M.
Department of Cellular Biochemistry, MPI for biophysical chemistry, Max Planck Society;

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Will,  C. L.
Department of Cellular Biochemistry, MPI for biophysical chemistry, Max Planck Society;

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Anokhina,  M.
Department of Cellular Biochemistry, MPI for biophysical chemistry, Max Planck Society;

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Urlaub,  H.
Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society;

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Lührmann,  R.
Department of Cellular Biochemistry, MPI for biophysical chemistry, Max Planck Society;

Fulltext (public)

587713.pdf
(Publisher version), 2MB

Supplementary Material (public)

587713-Suppl.pdf
(Supplementary material), 529KB

Citation

Schneider, M., Will, C. L., Anokhina, M., Tazi, J., Urlaub, H., & Lührmann, R. (2010). Exon definition complexes contain the tri-snRNP and can be directly converted into B-like precatalytic splicing complexes. Molecular Cell, 38(2), 223-235. doi:10.1016/j.molcel.2010.02.027.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0012-D5C1-C
Abstract
The first step in splicing of pre-mRNAs with long introns is exon definition, where U1 and U2 snRNPs bind at opposite ends of an exon. After exon definition, these snRNPs must form a complex across the upstream intron to allow splicing catalysis. Exon definition and conversion of cross-exon to cross-intron spliceosomal complexes are poorly understood. Here we demonstrate that, in addition to U1 and U2 snRNPs, cross-exon complexes contain U4, U5, and U6 (which form the tri-snRNP). Tri-snRNP docking involves the formation of U2/U6 helix II. This interaction is stabilized by a 5′ splice site (SS)-containing oligonucleotide, which can bind the tri-snRNP and convert the cross-exon complex into a cross-intron, B-like complex. Our data suggest that the switch from cross-exon to cross-intron complexes can occur directly when an exon-bound tri-snRNP interacts with an upstream 5′SS, without prior formation of a cross-intron A complex, revealing an alternative spliceosome assembly pathway.