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Effects in 94 GHz orientation-selected PELDOR on a rigid pair of radicals with non-collinear axes.

MPS-Authors
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Sicoli,  G.
Research Group of Electron Paramagnetic Resonance, MPI for biophysical chemistry, Max Planck Society;

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Argirevic,  T.
Research Group of Electron Paramagnetic Resonance, MPI for biophysical chemistry, Max Planck Society;

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Tkach,  I.
Research Group of Electron Paramagnetic Resonance, MPI for biophysical chemistry, Max Planck Society;

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Bennati,  M.
Research Group of Electron Paramagnetic Resonance, MPI for biophysical chemistry, Max Planck Society;

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Citation

Sicoli, G., Argirevic, T., Stubbe, J., Tkach, I., & Bennati, M. (2010). Effects in 94 GHz orientation-selected PELDOR on a rigid pair of radicals with non-collinear axes. Applied Magnetic Resonance, 37(1-4), 539-548. doi:10.1007/s00723-009-0094-3.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0012-D6F4-3
Abstract
For aromatic organic radicals, pulsed electron-electron double resonance (PELDOR) experiments at high magnetic fields provide information not only about the distance between the paramagnetic species but also about their relative orientation. However, the three-dimensional biradical structure is encoded in a complex pattern of orientation-selected PELDOR traces and the execution of the experiment is generally aggravated by constraints posed by the available hardware and the intrinsically low modulation depth observed. We present a 94 GHz PELDOR experiment performed with a commercial spectrometer and probe heads that permit separation of pump and detection frequencies up to 150 MHz. The setup is employed to examine the orientation selections on a general case of rigid biradicals with non-collinear g axes. The interacting radicals, a tyrosyl radical (Y122·) located in the β2 subunit and an 3-aminotyrosyl radical (NH2Y731·) located in the α2 subunit, are generated by Escherichia coli ribonucleotide reductase with a 3-aminotyrosine (NH2Y) site specifically incorporated into α2 in the presence of cytidine 5′-diphosphate and adenosine 5′-triphosphate. The experimental designs as well as some characteristic features of the observed modulation pattern are discussed.