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Journal Article

Differentiation of embryonic stem cells.

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Mansouri,  A.
Research Group of Molecular Cell Differentiation, MPI for biophysical chemistry, Max Planck Society;

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Schindehuette,  J.
Research Group of Molecular Cell Differentiation, MPI for biophysical chemistry, Max Planck Society;

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Citation

Mansouri, A., Fukumitsu, H., Schindehuette, J., & Krieglstein, K. (2009). Differentiation of embryonic stem cells. Current Protocols in Neuroscience, 47(Unit 3.6), 3.6.1-3.6.21. doi:10.1002/0471142301.ns0306s47.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0012-D89A-E
Abstract
Mouse embryonic stem (ES) cells are derived from mouse blastocyst and are able to generate all embryonic tissues in vitro. This propensity of ES cells has acquired considerable attention in recent years due to the promising potential for future cell replacement–based therapies. Therefore, it is of fundamental interest to establish protocols that allow the differentiation of ES cells into specific cell types. In recent years, several such differentiation procedures have been described for mouse and human embryonic stem cells. This unit describes a simple procedure that promotes the neuronal differentiation of mouse embryonic stem cells and yields a high proportion of midbrain dopaminergic neurons. Furthermore, this procedure permits the isolation of neural stem cell lines from mouse ES cells.