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Imaging quantum dots switched on and off by photochromic Fluorescence Resonance Energy Transfer (pcFRET)

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Jares-Erijman,  E. A.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Spagnuolo,  C.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Lidke,  K. A.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Jovin,  T. M.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Citation

Jares-Erijman, E. A., Giordano, L., Spagnuolo, C., Lidke, K. A., & Jovin, T. M. (2005). Imaging quantum dots switched on and off by photochromic Fluorescence Resonance Energy Transfer (pcFRET). Molecular Crystals and Liquid Crystals, 430: DOI: 10.1080/15421400590946479, pp. 257-265. Retrieved from http://www.informaworld.com/smpp/ftinterface~content=a713989262~fulltext=713240928.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0012-E8F6-A
Abstract
The reversible modulation of the emission of CdSe=ZnS semiconductor nanocrystals (quantum dots) was achieved by binding photochromic diheteroarylethenesand switchable acceptors for fluorescence resonance energy transfer. A biotinylated diheteroarylethene derivative was bound to quantum dots bearing conjugated streptavidin, leading to an intensity decrease as a consequence of energy transfer to the closed form of the acceptor. Interconversion between the open and closed forms by irradiation with 365 and 546nm light enabled deactivation and activation, respectively, of the FRET process with a corresponding modulation of quantum dot emission, observed both in solution and by sequential wide-field imaging.