English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Functional analysis of seven genes encoding eight translation initiation factor 4E (eIF4E) isoforms in Drosophila

MPS-Authors
/persons/resource/persons15218

Hernandez,  G.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15947

Urlaub,  H.
Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15657

Rivera-Pomar,  R.
Department of Cellular Biochemistry, MPI for biophysical chemistry, Max Planck Society;

Locator
There are no locators available
Fulltext (public)

214533.pdf
(Publisher version), 0B

Supplementary Material (public)
There is no public supplementary material available
Citation

Hernandez, G., Altmann, M., Sierra, J. M., Urlaub, H., Diez del Corral, R., Schwartz, P., et al. (2005). Functional analysis of seven genes encoding eight translation initiation factor 4E (eIF4E) isoforms in Drosophila. Mechanisms of Development, 122: doi:10.1016/j.mod.2004.11.011, pp. 529-543. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T9H-4F00RD2-1-C&_cdi=5115&_user=38661&_orig=browse&_coverDate=04%2F30%2F2005&_sk=998779995&view=c&wchp=dGLzVlz-zSkzV&md5=cdb88a9698452461508ca0f6f143a28b&ie=/sdarticle.pdf.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0012-E99B-1
Abstract
The Drosophila genome-sequencing project has revealed a total of seven genes encoding eight eukaryotic initiation factor 4E (eIF4E) isoforms. Four of them (eIF4E-1,2, eIF4E-3, eIF4E-4 and eIF4E-5) share exon/intron structure in their carboxy-terminal part and form a cluster in the genome. All eIF4E isoforms bind to the cap (m⁷GpppN) structure. All of them, except eIF4E-6 and eIF4E-8 were able to interact with Drosophila eIF4G or eIF4E-binding protein (4E-BP). eIF4E-1, eIF4E-2, eIF4E-3, eIF4E-4 and eIF4E-7 rescued a yeast eIF4Edeficient mutant in vivo. Only eIF4E-1 mRNAs and, at a significantly lower level, eIF4E3 and eIF4E-8 are expressed in embryos and throughout the life cycle of the fly. The transcripts of the remaining isoforms were detected from the third instar larvae onwards. This indicates the cap-binding activity relies mostly on eIF4E-1 during embryogenesis. This agrees with the proteomic analysis of the eIF4F complex purified from embryos and with the rescue of l(3)67Af, an embryonic lethal mutant for the eIF4E-1,2 gene, by transgenic expression of eIF4E-1. Overexpression of eIF4E-1 in wild-type embryos and eye imaginal discs results in phenotypic defects in a dose-dependent manner.