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Novel single cell fluorescence approaches in the investigation of signaling at the cellular level

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Nagy,  P.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Vereb,  G.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Post,  J. N.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Citation

Nagy, P., Vereb, G., Post, J. N., Friedlaender, E., & Szoelloesi, J. (2005). Novel single cell fluorescence approaches in the investigation of signaling at the cellular level. In S. Damjanovich (Ed.), Biophysical aspects of transmembrane signaling (pp. 33-70). Berlin; Heidelberg; New York: Springer.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0012-EA9A-A
Abstract
(from the Introduction:) In this chapter we give a brief overview of the application of fluorescence in quantitative cell biological studies, with special emphasis on Förster-type fluorescence resonance energy transfer (FRET) and intracellular trafficking, and address the advantages and potential pitfalls of different detection and labeling strategies from the standpoint of a cell or molecular biologist.