Gain-of-Function Screen for Genes That Affect Drosophila Muscle Pattern 
Formation

Staudt, N.; Molitor, A.; Somogyi, K.; Mata, J.; Curado, S.; Eulenberg, K. G.; Meise, M.; Siegmund, T.; Haeder, T.; Hilfiker, A.; Broenner, G.; Ephrussi, A.; Rroth, P.; Cohen, S. M.; Fellert, S.; Chung, H. R.; Piepenburg, O.; Schaefer, U.; Jäckle, H.; Vorbrüggen, G.

doi:10.1371/journal.pgen.0010055

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Gain-of-Function Screen for Genes That Affect Drosophila Muscle Pattern Formation

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Staudt, N.
Department of Molecular Developmental Biology, MPI for biophysical chemistry, Max Planck Society;

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Molitor, A.
Department of Molecular Developmental Biology, MPI for biophysical chemistry, Max Planck Society;

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Haeder, T.
Department of Molecular Developmental Biology, MPI for biophysical chemistry, Max Planck Society;

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Broenner, G.
Department of Molecular Developmental Biology, MPI for biophysical chemistry, Max Planck Society;

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Fellert, S.
Department of Molecular Developmental Biology, MPI for biophysical chemistry, Max Planck Society;

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Chung, H. R.
Department of Molecular Developmental Biology, MPI for biophysical chemistry, Max Planck Society;

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Piepenburg, O.
Department of Molecular Developmental Biology, MPI for biophysical chemistry, Max Planck Society;

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Schaefer, U.
Department of Molecular Developmental Biology, MPI for biophysical chemistry, Max Planck Society;

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Jäckle, H.
Department of Molecular Developmental Biology, MPI for biophysical chemistry, Max Planck Society;

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Vorbrüggen, G.
Research Group of Molecular Cell Dynamics, MPI for biophysical chemistry, Max Planck Society;

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Citation

Staudt, N., Molitor, A., Somogyi, K., Mata, J., Curado, S., Eulenberg, K. G., et al. (2005). Gain-of-Function Screen for Genes That Affect Drosophila Muscle Pattern Formation. PLoS Genetics, 1(4): e55. doi:10.1371/journal.pgen.0010055.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0012-EAD0-E

Abstract

This article reports the production of an EP-element insertion library with more than 3,700 unique target sites within the Drosophila melanogaster genome and its use to systematically identify genes that affect embryonic muscle pattern formation. We designed a UAS/GAL4 system to drive GAL4-responsive expression of the EP-targeted genes in developing apodeme cells to which migrating myotubes finally attach and in an intrasegmental pattern of cells that serve myotubes as a migration substrate on their way towards the apodemes. The results suggest that misexpression of more than 1.5% of the Drosophila genes can interfere with proper myotube guidance and/or muscle attachment. In addition to factors already known to participate in these processes, we identified a number of enzymes that participate in the synthesis or modification of protein carbohydrate side chains and in Ubiquitin modifications and/or the Ubiquitin-dependent degradation of proteins, suggesting that these processes are relevant for muscle pattern formation.