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Ensemble and single particle photophysical properties (Two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells

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Lidke,  K. A.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Heintzmann,  R.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Lidke,  D. S.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Spagnuolo,  C.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Jares-Erijman,  E. A.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Jovin,  T. M.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Citation

Grecco, H. E., Lidke, K. A., Heintzmann, R., Lidke, D. S., Spagnuolo, C., Martinez, O. E., et al. (2004). Ensemble and single particle photophysical properties (Two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells. Miscroscopy Research and Technique, 65: DOI 10.1002/jemt.20129, pp. 169-179. Retrieved from http://www3.interscience.wiley.com/cgi-bin/fulltext/109861786/PDFSTART.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0012-EB6E-8
Abstract
In this work we characterized streptavidin conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (i) two-photon excitation; (ii) fluorescence lifetimes; (iii) ensemble and single QD emission anisotropy; (iv) QDs as donors for Förster resonance energy transfer (FRET); and (v) spectral conversion of QDs exposed to high intensity illumination. We also demonstrate the utility of QDs for (i) imaging the binding and uptake of biotinylated transferrin on living cells, and (ii) resolving by fluorescence lifetime imaging microscopy (FLIM) signals originating from QDs from those of spatially and spectrally overlapping visible fluorescent proteins (VFPs).