Help Privacy Policy Disclaimer
  Advanced SearchBrowse




Journal Article

Diffusion of microspheres in sealed and open microarrays


Rieger,  B.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available

Rieger, B., Dietrich, H. R. C., van den Doel, R. L., & van Vliet, L. J. (2004). Diffusion of microspheres in sealed and open microarrays. Microscopy Research and Technique, 65: DOI 10.1002/jemt.20128, pp. 218-225. Retrieved from http://www3.interscience.wiley.com/cgi-bin/fulltext/109861802/PDFSTART.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0012-EB92-1
In several experiments, we study the diffusion of microspheres with different radii in microarrays filled with a variety of aqueous solutions of ethylene glycol. We study diffusion in open and closed (sealed) microarrays. In sealed nanoliter wells, the tracers show pure diffusion, whereas in open reactors, a radial outward-directed evaporation-induced liquid flow is superimposed onto the diffusion. In general, one of the following quantities can be calculated if the others are known: the temperature, the viscosity of the medium, the radius of the microbeads, or the diffusion constant. The estimated diffusion constants in closed microarrays are in good agreement with theoretical predictions based on the Brownian motion. We monitor the motion of the microbeads under a microscope and extract their paths in time from the digital recordings. Ambiguous paths due to the crossing of two trajectories can be detected. We show that low microsphere concentrations or high viscosities do not hamper a robust estimation of the diffusion parameters.