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Two functionally redundant isoforms of Drosophila melanogaster eukaryotic initiation factor 4B are involved in cap-dependent translation, cell survival and proliferation

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Hernandez,  G.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Vazquez-Pianzola,  P.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Rivera-Pomar,  R.
Department of Cellular Biochemistry, MPI for biophysical chemistry, Max Planck Society;

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Hernandez, G., Vazquez-Pianzola, P., Zurbriggen, M., Altmann, M., Sierra, J. M., & Rivera-Pomar, R. (2004). Two functionally redundant isoforms of Drosophila melanogaster eukaryotic initiation factor 4B are involved in cap-dependent translation, cell survival and proliferation. European Journal of Biochemistry, 271(14): doi:10.1111/j.1432-1033.2004.04217.x, pp. 2923-2926. Retrieved from http://www3.interscience.wiley.com/cgi-bin/fulltext/118751380/HTMLSTART.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0012-ED0C-3
Abstract
Eukaryotic initiation factor (eIF) 4B is part of the protein complex involved in the recognition and binding of mRNA to the ribosome. Drosophila eIF4B is a single-copy gene that encodes two isoforms, termed eIF4B-L (52.2 kDa) and eIF4B-S (44.2 kDa), generated as a result of the alternative recognition of two polyadeynlation signals during transcription termination and subsequent alternative splicing of the two pre-mRNAs. Both eIF4B mRNAs and proteins are expressed during the entire embryogenesis and life cycle. The proteins are cytoplasmic with polarized distribution. The two isoforms bindRNAwith the same affinity. eIF4B-L and eIF4B-S preferentially enhance cap-dependent over IRES-dependent translation initiation in a Drosophila cellfree translation system. RNA interference experiments suggest that eIF4B is required for cell survival, although only a modest reduction in rate of protein synthesis is observed. Overexpression of eIF4B in Drosophila cells in culture and in developing eye imaginal discs promotes cell proliferation.