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Journal Article

FRET Imaging

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Jares-Erijman,  E. A.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Jovin,  T. M.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Citation

Jares-Erijman, E. A., & Jovin, T. M. (2003). FRET Imaging. Nature biotechnology, 21(11), 1387-1395. Retrieved from http://www.nature.com/nbt/journal/v21/n11/pdf/nbt896.pdf.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0012-EFE0-8
Abstract
Förster (or Fluorescence) Resonance Energy Transfer (FRET) generates fluorescence signals sensitive to molecular conformation, association, and separation in the 1-10 nm range. We introduce a new photophysical framework for the phenomenon and apply it to the wide range of FRET techniques adapted to imaging systems. A systematic catalogue of 21 such methods is presented, including several new approaches proposed as suitable prospects for implementation. We conclude with a brief discussion of selected probe issues and anticipated future developments extending from single molecule to live cell applications.