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Journal Article

Involvement of actin polymerization in vesicle recruitment at the calyx of Held synapse

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Sakaba,  T.
Research Group of Biophysics of Synaptic Transmission, MPI for biophysical chemistry, Max Planck Society;

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Neher,  E.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Citation

Sakaba, T., & Neher, E. (2003). Involvement of actin polymerization in vesicle recruitment at the calyx of Held synapse. Journal of Neuroscience, 23(3), 837-846. Retrieved from http://www.jneurosci.org/cgi/reprint/23/3/837.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0012-F17F-6
Abstract
Depletion and replenishment of pools of synaptic vesicles are important determinants of short-term synaptic plasticity, but the underlying molecular mechanisms are not yet clear. As a first step toward understanding the process of vesicle recruitment, we have applied various specific agents directly to the presynaptic terminal of the calyx of Held synapse. Here we show that the nonhydrolyzable ATP analog ATP-gammaS retards the recovery from vesicle pool depletion, as does latrunculin A. Phalloidin has no effects on recovery, suggesting that dynamic actin reorganization is not necessary. Unexpectedly, neither N-ethylmaleimide nor staurosporine affected the recovery, calling into question the role of N-ethylmaleimide- sensitive factor and protein kinases. The results suggest that intact actin polymerization is involved in vesicle recruitment.