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Journal Article

Sr2+ binding to the Ca2+ binding site of the synaptotagmin 1 C2B domain triggers fast exocytosis without stimulating SNARE interactions

MPS-Authors
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Rhee,  J. S.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Rosenmund,  C.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Citation

Shin, O. H., Rhee, J. S., Tang, J., Sugita, S., Rosenmund, C., & Suedhof, T. C. (2003). Sr2+ binding to the Ca2+ binding site of the synaptotagmin 1 C2B domain triggers fast exocytosis without stimulating SNARE interactions. Neuron, 37(1), 99-108. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WSS-47P8K6X-F-1&_cdi=7054&_user=38661&_pii=S0896627302011455&_orig=search&_coverDate=01%2F09%2F2003&_sk=999629998&view=c&wchp=dGLzVlb-zSkzS&md5=a356a79b204d8e4c7fb3b62e5be6473a&ie=/sdarticle.pdf.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0012-F1A2-1
Abstract
Sr2+ triggers neurotransmitter release similar to Ca2+, but less efficiently. We now show that in synaptotagmin 1 knockout mice, the fast component of both Ca2+- and Sr2+-induced release is selectively impaired, suggesting that both cations partly act by binding to synaptotagmin 1. Both the C(2)A and the C2B domain of synaptotagmin 1 bind Ca2+ in phospholipid complexes, but only the C2B domain forms Sr2+/phospholipid complexes; therefore, Sr2+ binding to the C2B domain is sufficient to trigger fast release, although with decreased efficacy. Ca2+ induces binding of the synaptotagmin C, domains to SNARE proteins, whereas Sr2+ even at high concentrations does not. Thus, triggering of the fast component of release by Sr2+ as a Ca2+ agonist involves the formation of synaptotagmin/ phospholipid complexes, but does not require stimulated SNARE binding.