Enhanced spectral resolution in immobilized peptides and proteins by combining 
chemical shift sum and difference spectroscopy

Luca, S.; Baldus, M.

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Enhanced spectral resolution in immobilized peptides and proteins by combining chemical shift sum and difference spectroscopy

MPS-Authors

/persons/resource/persons15467

Luca, S.
Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons14804

Baldus, M.
Research Group of Solid State NMR Spectroscopy, MPI for biophysical chemistry, Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

17180.pdf
(出版社版), 0B

付随資料 (公開)

There is no public supplementary material available

引用

Luca, S., & Baldus, M. (2002). Enhanced spectral resolution in immobilized peptides and proteins by combining chemical shift sum and difference spectroscopy. Journal of Magnetic Resonance, 159(2), 243-249. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WJX-479TFSJ-4-2B&_cdi=6890&_user=38661&_pii=S1090780702000198&_orig=search&_coverDate=12%2F31%2F2002&_sk=998409997&view=c&wchp=dGLbVlb-zSkzk&md5=0fb80dd870cbe7d0d84d3db72ed09452&ie=/sdarticle.pdf.

引用: https://hdl.handle.net/11858/00-001M-0000-0012-F273-3

要旨

A two-dimensional correlation experiment is introduced that records the sum and difference chemical shift of two scalar or dipolar coupled nuclei. Statistical results indicate that the suggested pulse scheme can significantly increase the possibility of separating chemical shift contributions due to residue type and backbone conformation in immobilized peptides and proteins. Experimental applications demonstrate the theoretical concept and lead to the predicted resolution enhancement between different amino acid types and among protein residues of different secondary structure. (C) 2002 Elsevier Science (USA). All rights reserved.