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High-resolution 3D MRI of mouse brain reveals small cerebral structures in vivo

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Natt,  O.
Biomedical NMR Research GmbH, MPI for biophysical chemistry, Max Planck Society;

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Watanabe,  T.
Biomedical NMR Research GmbH, MPI for biophysical chemistry, Max Planck Society;

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Boretius,  S.
Biomedical NMR Research GmbH, MPI for biophysical chemistry, Max Planck Society;

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Frahm,  J.
Biomedical NMR Research GmbH, MPI for biophysical chemistry, Max Planck Society;

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Michaelis,  T.
Biomedical NMR Research GmbH, MPI for biophysical chemistry, Max Planck Society;

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16693.pdf
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Citation

Natt, O., Watanabe, T., Boretius, S., Radulovic, J., Frahm, J., & Michaelis, T. (2002). High-resolution 3D MRI of mouse brain reveals small cerebral structures in vivo. Journal of Neuroscience Methods, 120(2), 203-209. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T04-46YV98M-C-G&_cdi=4852&_user=38661&_pii=S016502700200211X&_orig=search&_coverDate=10%2F30%2F2002&_sk=998799997&view=c&wchp=dGLbVlz-zSkzk&md5=0b87492f0f770a07ebb3747a8f5cbd79&ie=/sdarticle.pdf.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0012-F2AA-9
Abstract
This work demonstrates technical approaches to high-quality magnetic resonance imaging (MRI) of small structures of the mouse brain in vivo. It turns out that excellent soft-tissue contrast requires the reduction of partial volume effects by using 3D MRI at high (isotropic) resolution with linear voxel dimensions of about 100-150 mum. The long T-2* relaxation times at relatively low magnetic fields (2.35 T) offer the benefit of a small receiver bandwidth (increased signal-to-noise) at a moderate echo time which together with the small voxel size avoids visual susceptibility artifacts. For measuring times of 1-1.5 h both * relaxation times at relatively low magnetic fields (2.35 T) offer the benefit of a small receiver bandwidth (increased signal-to-noise) at a moderate echo time which together with the small voxel size avoids visual susceptibility artifacts. For measuring times of 1-1.5 h both T-1-weighted (FLASH) and T-2-weighted (Fast Spin-Echo) 3D MRI acquisitions exhibit detailed anatomical insights in accordance with histological sections from a mouse brain atlas. Preliminary applications address the identification of neuroanatomical variations in different mouse strains and the use of Mn2+ as a T-1 contrast agent for neuroaxonal tracing of fiber tracts within the mouse visual pathway. (C) 2002 Elsevier Science B.V. All rights reserved.