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Journal Article

Determination of the unknown phase difference in 4Pi-confocal microscopy through the image intensity

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Bewersdorf,  J.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Hell,  S. W.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Citation

Blanca, C. M., Bewersdorf, J., & Hell, S. W. (2002). Determination of the unknown phase difference in 4Pi-confocal microscopy through the image intensity. Optics Communications, 206(4-6), 281-285. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TVF-45PTYPJ-7-1R&_cdi=5533&_user=38661&_pii=S0030401802014244&_origin=search&_coverDate=06%2F01%2F2002&_sk=997939995&view=c&wchp=dGLbVzz-zSkzk&md5=d5d1654fc9c5392efb239604d6ad7c28&ie=/sdarticle.pdf.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0012-F399-7
Abstract
We investigate the effect of the phase difference of the interfering wavefronts on the image intensity in 4Pi-confocal microscopy and thereby introduce a method to diminish the problem of the unknown phase difference in this microscope. An efficient implementation of our method utilizes binary amplitude filters reshaping the point-spread-function such that a significant disparity arises between the maximum fluorescence intensity in the constructive and destructive interference case. In the presence of planar and point-like features in the sample this property enables the system to unambiguously determine the mode of interference. (C) 2002 Elsevier Science B.V. All rights reserved