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Journal Article

Dual-color 4Pi-confocal microscopy with 3D-resolution in the 100 nm range

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Jakobs,  S.
Research Group of High Resolution Optical Microscopy, MPI for Biophysical Chemistry, Max Planck Society;

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Nagorni,  M.
Research Group of High Resolution Optical Microscopy, MPI for Biophysical Chemistry, Max Planck Society;

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Hell,  S. W.
Research Group of High Resolution Optical Microscopy, MPI for Biophysical Chemistry, Max Planck Society;

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Citation

Kano, H., Jakobs, S., Nagorni, M., & Hell, S. W. (2002). Dual-color 4Pi-confocal microscopy with 3D-resolution in the 100 nm range. Ultramicroscopy, 90(2-3), 207-213. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TW1-443K184-1-G&_cdi=5549&_user=38661&_pii=S0304399101001322&_origin=search&_coverDate=02%2F28%2F2002&_sk=999099997&view=c&wchp=dGLbVtz-zSkzS&md5=d206ae2ac93f8cdbf9f687aab8c3205f&ie=/sdarticle.pdf.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0012-F457-4
Abstract
We report the development of simultaneous two-color channel recording in 4Pi-confocal microscopy. A marked increase of spatial resolution over confocal microscopy becomes manifested in 4Pi-confocal three-dimensional (313) data stacks of dual- labeled objects. The fundamentally improved resolution is verified both with densely labeled fluorescence beads as well as with membrane labeled fixed Escherichia coli. The synergistic combination of dual-color 4Pi-confocal recording with image restoration results in dual-color imaging with a 3D resolution in the 100 nm range. (C) 2002 Elsevier Science B.V. All rights reserved