English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

The telomeric dG(GT)4G sequence can adopt a parallel-stranded double helical conformation

MPS-Authors
/persons/resource/persons15339

Klement,  R.
Emeritus Group Laboratory of Cellular Dynamics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15286

Jovin,  T. M.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

Locator
There are no locators available
Fulltext (public)

600067.pdf
(Publisher version), 845KB

Supplementary Material (public)
There is no public supplementary material available
Citation

Shchyolkina, A. K., Borisova, O. F., Livshits, M. A., Klement, R., & Jovin, T. M. (2001). The telomeric dG(GT)4G sequence can adopt a parallel-stranded double helical conformation. Journal of Biomolecular Structure and Dynamics, 18, 493-503.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0012-F713-F
Abstract
Oligonucleotides 3'-d(GTGTGTGTGG)-L-d(GGTGTGTGTG)-3' (hp-GT) and 3'-d(G44SG4STG4STG4STGG)-L-d(GGTGTGTGTG)-3' (hp-SGT), (L=(CH2CH2O)3), were shown by use of several optical techniques to form a novel parallel-stranded (ps) intramolecular double helix with purine-purine and pyrimidine-pyrimidine base pairing. The rotational relaxation time of hp-GT was similar to that of a 10-bp reference duplex, and the fraction of unpaired bases was determined to be ~7%, testifying to the formation of an intramolecular double helical hairpin by the sequence under the given experimental conditions. A quasi-two-state mode of ps-double helix formation was validated, yielding a helix-coil transition enthalpy of -135±5 kJ/mol. The G·G and T·T (or 4ST ·T) base pair configurations and conformational parameters of the double helix were derived with molecular modeling by force field techniques. Repetitive d(GT) sequences are abundant in telomers of different genomes and in the regulatory regions of genes. Thus, the observed conformational potential of the repetitive d(GT) sequence may be of importance in the regulation of cell processes.