Fluorescence Correlation Microscopy (FCM) - Fluorescence Correlation 
Spectroscopy (FCS) taken into the cell

Brock, R.; Jovin, T. M.

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Fluorescence Correlation Microscopy (FCM) - Fluorescence Correlation Spectroscopy (FCS) taken into the cell

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Brock, R.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Jovin, T. M.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Citation

Brock, R., & Jovin, T. M. (1998). Fluorescence Correlation Microscopy (FCM) - Fluorescence Correlation Spectroscopy (FCS) taken into the cell. Cellular and Molecular Biology, 44, 847-856.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0012-FC70-6

Abstract

Confocal fluorescence correlation spectroscopy (FCS) and other confocal spectroscopic techniques are ideally suited for the analysis of molecular interactions at the subcellular level. However, one requires exact positioning in three dimensions within the cell. Our instrument integrates FCS with high sensitivity digital imaging microscopy and high precision positioning. We present first measurements of intracellular FCS, with specification of the instrumental requirements and methods of data analysis. We propose the term fluorescence correlation microscopy (FCM) for this extended modality of FCS.