English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Interferometric measurement of fluorescence excitation spectra

MPS-Authors
/persons/resource/persons15951

Vereb,  G.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15332

Kirsch,  A. K.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15286

Jovin,  T. M.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

Locator
There are no locators available
Fulltext (public)
There are no public fulltexts available
Supplementary Material (public)
There is no public supplementary material available
Citation

Hirschberg, J. G., Vereb, G., Meyer, C. K., Kirsch, A. K., Kohen, E., & Jovin, T. M. (1998). Interferometric measurement of fluorescence excitation spectra. Applied Optics, 37, 1953-1957.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0012-FCFF-A
Abstract
A Michelson interferometer has been adapted as an excitation source for fluorescence spectroscopy. A moving fringe pattern was generated by linear displacement of the movable mirror of the Michelson interferometer coupled to a xenon-arc lamp. This spectrally modulated source was monitored by a reference photomultiplier and used for exciting a Rhodamine B solution. The fluorescence emission at >645 nm was detected by a second photomultiplier. The two interferograms were acquired by a dual-channel digital oscilloscope, and their discrete Fourier transforms and corresponding power spectra were generated in a computer. The power spectrum of the emission signal represented the excitation spectrum, as was confirmed by comparison with the absorption spectrum of Rhodamine B. This optical arrangement is well suited for acquiring fluorescence excitation spectra in the optical microscopy of biological specimens.