Low mobility of the Ca2+ buffers in axons of cultured Aplysia neurons

Gabso, M.; Neher, E.; Spira, M. E.

doi:10.1016/S0896-6273(00)81247-7

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Low mobility of the Ca²⁺ buffers in axons of cultured Aplysia neurons

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Neher, E.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Citation

Gabso, M., Neher, E., & Spira, M. E. (1997). Low mobility of the Ca²⁺ buffers in axons of cultured Aplysia neurons. Neuron, 18(3), 473-481. doi:10.1016/S0896-6273(00)81247-7.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0012-FFF8-2

Abstract

Cellular Ca2+ buffers determine amplitude and diffusional spread of neuronal Ca2+ signals. Fixed Ca2+ buffers tend to retard the signal and to lower the apparent diffusion coefficient (Dapp) of Ca2+, whereas mobile buffers contribute to Ca2+ redistribution. To estimate the impact of the expression of specific Ca2+-binding proteins or the errors in Ca2+ measurement introduced by indicator dyes, the diffusion coefficient De and the Ca2+-binding ratio κe of endogenous Ca2+ buffers must be known. In this study, we obtain upper bounds to these quantities (De < 16 μm2/s; κe < 60) for axoplasm of metacerebral cells of Aplysia california. Due to these very low values, even minute concentrations of indicator dyes will interfere with the spatiotemporal pattern of Ca2+ signals and will conceal changes in the expression of specific Ca2+-binding proteins, which in the native neuron are expected to have significant effects on Ca2+ signals.